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Figure 4. Protein tree of HpnR, the C-3 methylase that methylates BHPs to form 3-MeBHP. Both Rhodopila strain LVNP and Rhodopila globiformis strain DSM 161T genomes encode HpnR, and the amino acid sequences of this protein cluster with those from their close phylogenetic relatives, the acetic acid bacteria. C-2 BHP methylase (HpnP) from Rhodopseudomonas palustris was used as the outgroup in the tree. Numbers at the nodes are bootstrap percentages based on 1000 replications.